Custom Radiolabeling of Synthetic Oligonucleotides
AM Chemicals offers services in expert synthesis of radiolabeled oligonucleotides and their phosphorothioate analogs (1 to 100 µmol of isolated product) including:
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Synthesis of -labeled, protected nucleoside phosphoramidite building blocks
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Synthesis of the target radiolabeled oligonucleotides
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Purification by reverse-phase or ion-exchange HPLC as requested by customer and desalting
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For siRNA, forming a duplex by hybridization with the unlabeled strand
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Optionally, confirmation of structure by ES MS
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Optionally, removal of endotoxin from the final product
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Optionally, performing above operations under GMP conditions
Custom Quote
AM Chemicals is pleased to provide a custom quote for radiolabeled oligonucleotide synthesis project.
To request the custom quote, please furnish the following information:
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Oligonucleotide sequence
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Desired radioisotope ( )
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Placement of the radiolabeled nucleoside in the sequence
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Desired specific activity (SA):
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A single labeling affords SA of 30-40, 100-400, and 500-1500 Ci/mol for radioisotopes, respectively
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Desired amount of radiolabel incorporated or amount of radiolabeled oligonucleotide
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Customers’ ability to provide project-specific unlabeled phosphoramidites of modified nucleosides or non-nucleosidic moieties
Guidelines for Optimal Cost Control of Radio-labeling Project
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Awareness of radiolytic degradation saves money: aim for the specific activity of the target oligonucleotide as low as permissible
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Use -thymidine or as carriers of the radiolabel
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Placement of the radiolabeled moiety: close to the 5'-terminus of the target oligonucleotide
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Provide project-specific unlabeled phosphoramidites of nucleosides or non-nucleosidic moieties
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For shipping, solution is a preferred physical state of the finished product (oligo)
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Acknowledge lead time for radio-labeling project (8 to 12 weeks) and plan ahead
