DMT-Protected Universal Solid Supports

The hydroxy groups in the supports above are protected with DMT and either acetyl group or succinate
linker. In the chemical environment of the universal linker, the DMT group is more stable than that at the 5’-
terminus of oligonucleotides.  An extended detritylation time (4 min) is required for the complete detritylation.  
The extended detritylation step should only be used in the first cycle of the chain assembly.  A more
prolonged detritylation of nucleoside residues is neither necessary nor advisable.

There are two types of universal solid supports different in the attachment of the universal linker to the solid

In the first type, the universal linker is attached via a cleavable ester bond of succinyl linker.  These supports
(products 01211-01213 and 01231-01233) are perfect for small-scale applications including the synthesis in
96-well plates.  The
N-iPr supports p/n 01231-01233 are our latest addition to the family and are superior t
the older version with labile C3 linker (p/n 01211-01213). When labile linkers are used, the oligonucleotides
are first released from the solid phase.  With ammonia-methylamine mixture (AMA) and concentrated
aqueous ammonium hydroxide at room temperature, this step requires 15 min and 1 h, respectively.  The
dephosphorylation and the base deprotection are then carried out in solution.  The complete
dephosphorylation of P=O 2’-deoxyoligonucleotides assembled on
N-iPr supports p/n 01231-01233  with
AMA and concentrated aqueous ammonium hydroxide requires 45 min and 6 h at 65°C, respectively.  With
gaseous ammonia, following the standard protocol for the deprotection of nucleic bases is sufficient for the
completion of the 3'-dephosphorylation.  With 2'-O-substituted nucleosides, the dephosphorylation is more
rapid.  Importantly, the dephosphorylation of the 3'-terminus is more rapid than the deprotection of nucleic
bases even when the labile base-protecting groups are used.  When P=O 2'-deoxyoligonucleotides are
assembled on the supports p/n 01211-01213, dephosphorylation with AMA and conc. aqueous ammonium
hydroxide requires 1 h and 8 h at 65
°C, respectively.

In the second type of solid supports (items 01221-01223), the universal linker is attached to the solid phase
via a chemically inert amide bond.  In the final deprotection step, the solid phase should be kept in contact
with the deprotecting agent over a period of time sufficient to complete the 3'-dephosphorylation (see
above).  These supports are most suitable for large-scale applications where the time of exposure of the
solid phase to concentrated aqueous ammonium hydroxide is less critical.

To read and download the User Guide for Universal Solid Supports, use
this link.
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DMT-protected universal solid supports
with labile C3 linker  
Items 01211-01213
DMT-protected universal solid supports
with a stable linker
Items 01221-01223
DMT-protected universal solid supports
with labile
N-iPr linker   
Items 01231-01233
DMT-protected universal solid support
with labile
N-Me linker   
can be purchased from Glen Research Corp.